what advantages does SEM have over the TEM?
what advantages does SEM have over the TEM.
and what advantages does TEM have over the SEM
if u dont know what im talking obout im reffering to cells.
please answer! easy points!
A SEM (scanning electron microscope) images using the electrons reflected from a specimen. A TEM (transmission electron microscope) images using the electrons that pass through it.
The image from an SEM thus looks somewhat like a normal photo (we’re used to imaging using the light reflected from objects). However, a TEM image takes a bit more interpretation as we’re not used to seeing images of light that’s passed through things – think of silhouettes or slide projectors…
The TEM transmits electrons through a sample that has been cut so that it is only a few molecules thin. Indeed, the sample is so thin that the electrons have enough energy to pass right through some regions of the sample. In other regions, where metals that were added to the sample have bound to sample molecules, the electrons either do not pass through as easily, or are restricted from passing through altogether. The different behaviors of the electrons are detected on special film that is positioned on the opposite side of the sample from the electron source.
Because TEM uses slices of a sample, it reveals internal details of a sample. In SEM, the electrons do not penetrate the sample. Rather, the sample is coated with gold, which causes the electrons to bounce off of the surface of the sample. The electron beam is scanned in a back and forth motion parallel to the sample surface. A detector captures the electrons that have bounced off the surface, and the pattern of deflection is used to assemble a three dimensional image of the sample surface.
Originally posted 2010-02-04 16:26:24.
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